644 research outputs found
STUDIES ON PROTEIN UPTAKE BY ISOLATED TUMOR CELLS : II. Quantitative Data on the Adsorption and Uptake of I131-Serum Albumin by Ehrlich Ascites Tumor Cells
Surface adsorption is studied in some detail because it is believed to be a major artifact in measurements of protein uptake by mammalian cells. Adsorption increases linearly with the I131-albumin concentration between 0.001 and 300 mg/ml. After short exposure to 300 mg/ml and two cell washings, the adsorption amounts to 38 mg albumin per gm cell proteins. Further washings remove 80 per cent of this value, leaving a small irreversibly bound residue. At equilibrium, adsorbed albumin can be labeled by a simple albumin exchange. This labeling reaches a steady state within seconds and stays at constant level over 30 minutes. Significant increases above this initial level are measured over periods of 2 hours. In our experimental conditions these increases can be considered due to albumin uptake. This uptake rises linearly with the albumin concentration between 0.5 and 50.0 mg/ml, and reaches 0.2 mg/gm cell protein or 4 x 105 molecules per cell. Compared to the incorporation of free amino acids in similar conditions, this value does not appear to contribute significantly to the N-metabolism of the tumor cells. Adsorption was generally greater than uptake. Both processes are linear functions of the same variable over the whole range of concentration tested. It is suggested that albumin is taken up by pinocytosis
STUDIES ON PROTEIN UPTAKE BY ISOLATED TUMOR CELLS : I. Electron Microscopic Evidence of Ferritin Uptake by Ehrlich Ascites Tumor Cells
Ferritin, added to the incubation medium of ascites tumor cells, was used as an electron microscopic marker to study the uptake of large protein molecules by morphologically intact cells. A definite uptake could be detected after 1 hour of incubation in Tyrode bicarbonate solution containing 0.04 to 13.3 mg ferritin/ml. Ferritin was found in a variety of membrane-surrounded structures, suggesting that pinocytesis and related membrane movements are occurring under physiological conditions and can account for the penetration of intact macromolecules into isolated tumor cells. Supplementation of the medium with serum albumin (33 mg/ml) increased the average amount of ferritin per cell and per pinocytotic structure. Ferritin was strongly adsorbed by fragments of lysed cells, which were readily taken up by intact cells. Besides its role as carrier, this debris appeared to stimulate membrane movements. Only rare examples were found to suggest the release of ferritin from the pinocytotic structures into the cytoplasm. Thus, the disintegration of such structures cannot be considered an obvious step towards a rapid metabolic utilization of protein by the cell. Particles of colloidal gold presented to the cell under the same conditions were not taken up to any significant extent, thus providing good evidence for a selective ingestion of particles of comparable sizes
Zero-Transmission Law for Multiport Beam Splitters
The Hong-Ou-Mandel effect is generalized to a configuration of n bosons
prepared in the n input ports of a Bell multiport beam splitter. We derive a
strict suppression law for most possible output events, consistent with a
generic bosonic behavior after suitable coarse graining.Comment: Version accepted by PR
Matrix permanent and quantum entanglement of permutation invariant states
We point out that a geometric measure of quantum entanglement is related to
the matrix permanent when restricted to permutation invariant states. This
connection allows us to interpret the permanent as an angle between vectors. By
employing a recently introduced permanent inequality by Carlen, Loss and Lieb,
we can prove explicit formulas of the geometric measure for permutation
invariant basis states in a simple way.Comment: 10 page
Rainbow matchings in Dirac bipartite graphs
This is the peer reviewed version of the following article: Coulson, M, Perarnau, G. Rainbow matchings in Dirac bipartite graphs. Random Struct Alg. 2019; 55: 271– 289., which has been published in final form at https://doi.org/10.1002/rsa.20835. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived VersionsWe show the existence of rainbow perfect matchings in µn-bounded edge colorings of Dirac bipartite graphs, for a sufficiently small µ¿>¿0. As an application of our results, we obtain several results on the existence of rainbow k-factors in Dirac graphs and rainbow spanning subgraphs of bounded maximum degree on graphs with large minimum degree
Link and subgraph likelihoods in random undirected networks with fixed and partially fixed degree sequence
The simplest null models for networks, used to distinguish significant
features of a particular network from {\it a priori} expected features, are
random ensembles with the degree sequence fixed by the specific network of
interest. These "fixed degree sequence" (FDS) ensembles are, however, famously
resistant to analytic attack. In this paper we introduce ensembles with
partially-fixed degree sequences (PFDS) and compare analytic results obtained
for them with Monte Carlo results for the FDS ensemble. These results include
link likelihoods, subgraph likelihoods, and degree correlations. We find that
local structural features in the FDS ensemble can be reasonably well estimated
by simultaneously fixing only the degrees of few nodes, in addition to the
total number of nodes and links. As test cases we use a food web, two protein
interaction networks (\textit{E. coli, S. cerevisiae}), the internet on the
autonomous system (AS) level, and the World Wide Web. Fixing just the degrees
of two nodes gives the mean neighbor degree as a function of node degree,
, in agreement with results explicitly obtained from rewiring. For
power law degree distributions, we derive the disassortativity analytically. In
the PFDS ensemble the partition function can be expanded diagrammatically. We
obtain an explicit expression for the link likelihood to lowest order, which
reduces in the limit of large, sparse undirected networks with links and
with to the simple formula . In a
similar limit, the probability for three nodes to be linked into a triangle
reduces to the factorized expression .Comment: 17 pages, includes 11 figures; first revision: shortened to 14 pages
(7 figures), added discussion of subgraph counts, deleted discussion of
directed network
Decision and function problems based on boson sampling
Boson sampling is a mathematical problem that is strongly believed to be
intractable for classical computers, whereas passive linear interferometers can
produce samples efficiently. So far, the problem remains a computational
curiosity, and the possible usefulness of boson-sampling devices is mainly
limited to the proof of quantum supremacy. The purpose of this work is to
investigate whether boson sampling can be used as a resource of decision and
function problems that are computationally hard, and may thus have
cryptographic applications. After the definition of a rather general
theoretical framework for the design of such problems, we discuss their
solution by means of a brute-force numerical approach, as well as by means of
non-boson samplers. Moreover, we estimate the sample sizes required for their
solution by passive linear interferometers, and it is shown that they are
independent of the size of the Hilbert space.Comment: Close to the version published in PR
Many-particle interference beyond many-boson and many-fermion statistics
Identical particles exhibit correlations even in the absence of
inter-particle interaction, due to the exchange (anti)symmetry of the
many-particle wavefunction. Two fermions obey the Pauli principle and
anti-bunch, whereas two bosons favor bunched, doubly occupied states. Here, we
show that the collective interference of three or more particles leads to a
much more diverse behavior than expected from the boson-fermion dichotomy known
from quantum statistical mechanics. The emerging complexity of many-particle
interference is tamed by a simple law for the strict suppression of events in
the Bell multiport beam splitter. The law shows that counting events are
governed by widely species-independent interference, such that bosons and
fermions can even exhibit identical interference signatures, while their
statistical character remains subordinate. Recent progress in the preparation
of tailored many-particle states of bosonic and fermionic atoms promises
experimental verification and applications in novel many-particle
interferometers.Comment: 12 pages, 5 figure
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